Cell Cycle And Dna Rn Mirnas Regulating Cellular...

I am a young researcher from India aspiring for a PhD position. I obtained my master degree in biochemistry from Osmania University, India. Currently I’m a senior research fellow in the laboratory of DNA Replication and Cell Cycle at National Institute of Immunology, India, under the supervision of Dr. Sandeep Saxena. The main focus of the lab is to study the role of non-coding RNAs in regulation of Cell Cycle and DNA replication and also to provide mechanical insights in understanding the checkpoint response to aberrations in replication complexes. My project work is focused on understanding the role of non-coding RNA – miRNAs regulating cellular proliferation during oncogenic transformations. miRNA targeting mRNA regulates gene expression post transcriptionally and plays an important role in tumor progression. To identify differentially expressed miRNAs in Osteosarcoma cell lines which have different proliferation rates, I have screened miRNAs from relevant microRNA datasets and upregulated and downregulated microRNAs were shortlisted and their expression were analyzed in the respective OS cell line by real-time RT PCR. Potential targets for miRNAs were screened for cell cycle and DNA replication genes by target scan and KEGG database. Downregulated miRNAs were overexpressed in OS cell lines by transfecting the synthetic mimics/ inhibitors and checked the expression levels of the target protein at RNA level by qRT PCR and at protein level by Immunoblotting. Further cell